Fig 1: Taenia solium GDH and IDH license Treg induction from naïve CD4+ T cells via PGE2 and IL-10 secretion Induction of Treg following PBMC treatment with either native or proteinase K-treated CLys or CSN (10 µg/ml) for 72 h.Mass spectrometry analysis of CVF, CLys, and CSN and identification of distinct and shared proteins. From the shared proteins in CLys and CSN, relative detection scores of GDH and IDH were presented.PBMCs were treated with GDH inhibitor bithionol (BT) (20 µM) or IDH inhibitor IDH-305 (50 nM) alone or in combination before incubation with CLys or CSN. Percentage of Treg was determined and PGE2 and IL-10 levels were evaluated.Treg induction from PBMC by CLys, CSN, and TsGDH (5 µg/ml) and levels of PGE2 and IL-10 in culture supernatants.Treg induction from monocyte-naïve CD4+-TsGDH culture and levels of PGE2 and IL-10 in monocyte-CD4+-TsGDH and monocyte-TsGDH culture supernatants.Treg induction from PBMC by CLys, CSN, and rhGDH (10 µg/ml). Data information: Graphs are representative of 5–7 biological replicates (n = 5–7) for (A-E) and 8 biological replicates (n = 8) for (F). Results are expressed as means ± SEM. Asterisks show significant statistical differences analyzed using Mann–Whitney U test (A) or Kruskal–Wallis one-way ANOVA followed by a Dunn’s multiple comparison test (C). *P < 0.05; **P < 0.01; ***P < 0.001.